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Beilstein J. Org. Chem. 2013, 9, 2168–2179, doi:10.3762/bjoc.9.254
Graphical Abstract
Scheme 1: Phenylserine synthesis.
Figure 1: Activity loss of TA immobilized by two different methods and as a free enzyme at 80 °C. Reproduced ...
Figure 2: Degree of immobilization versus incubation time.
Figure 3: Batch reaction using free enzyme. Reaction conditions: Reaction volume (10 mL), TA (2.7 mg, specifi...
Figure 4: Product inhibition study.
Figure 5: Effect of temperature on product yield. Reaction conditions: Reaction volume (0.250 mL), TA (1.1 mg...
Figure 6: Effect of flow rates on yield for immobilized enzymes in a packed bed microreactor (70 °C). Reactio...
Figure 7: Effect of residence time on yield for free enzymes in a Teflon tube microreactor at 70 °C. Reaction...
Figure 8: Long term enzyme stability at 70 °C. Reaction conditions: Reaction volume (0.250 mL), TA (1.1 mg, s...
Scheme 2: Synthesis of chiral α-aminoalcohol by telescoping aldolase reaction with decarboxylation.
Figure 9: Direct immobilization.
Figure 10: Indirect immobilization.
Figure 11: Flow reaction set-up using free enzyme.
Figure 12: Experimental setup for packed be microreactor.
Figure 13: Analysis of the four isomers of phenylserine on a chiral column.